Chemical composition, polyphenol contents and antioxidant activities of the 'Himalayan toothache relieving tree' (Zanthoxylum armatum DC.).

The present study carried out to investigate the bioactive chemical compounds, total polyphenol content and antioxidant potential of different extracts of the Zanthoxylum armatum leaves collected from the Nainital, Uttarakhand. The GC-MS analysis of Z. armtum leaves extract resulted in the isolation of sixty, twelve, twenty-three and nineteen phytochemical constituents in methanol, ethanol, chloroform and water extracts respectively. The leaves extracts were strongly characterised by Heneicosane, Tetratetracontane, Phytol, Fargesin, (+)- Seasmin and Paulowin. Methanol extract showed maximum DPPH (2,2-Di-phenyl-1-picryl-hydrazyl) Free radical scavenging activity (IC 50 15.63 ± 0.31), Ferric Reducing Antioxidant Activity (88.98 ± 3.34 AAE ± SD) and Metal Chelating Activity (IC 50 9.89 ± 0.83). The results showed that the methanolic extract exhibited the highest phenolic content for total phenol content (98.26 ± 0.8 mg of Gallic acid equivalent/g of dry weight), total flavonoid content (61.50 ± 1.62 mg of Quercetin equivalent/g of dry weight) and total tannin content (79.96 ± 0.81 mg of Tannic acid equivalent/g of dry weight).