Biotechnologia · 2025
In vitro regeneration of Portulaca grandiflora Hook. and analysis of betalain content within in vivo plants.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Srivastava Archana, Joshi Aruna
Аннотация
BACKGROUND: Synthetic colors are widely used in the food and cosmetic industries to make products more appealing to consumers. However, the health hazards associated with synthetic colors have prompted their replacement with natural colors. Portulaca grandiflora is a promising candidate for natural color extraction, as it is rich in betalains. This study presents a reliable, reproducible three-step regeneration protocol for this plant and analyzes its betalain content.
MATERIALS AND METHODS: In vitro shoot cultures were established on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Anatomical studies were conducted to determine the stages of shoot primordium development. Betalains were extracted from in vivo plants using 60% methanol and subjected to spectrophotometric analysis. The effect of sodium ascorbate on betalain stability was also evaluated.
RESULTS: Juvenile leaf explants regenerated shoots on MS medium supplemented with 10 μM 6-benzyladenine and 5 μM indole-3-acetic acid. Shoots were multiplied with 20 μM BA (6.25 ± 0.85 shoots/explant), and elongation was achieved with 5 μM gibberellic acid (GA3) (8.2 ± 0.37 shoots/explant). Shoot primordia developed from well-organized meristemoid cells. The betalain content in the stem was 26.66 ± 0.19 mg/100 g, but this pigment degraded within 24 h (42.19% degradation). The addition of 50 mM sodium ascorbate prevented betalain degradation, even after 24 h.
CONCLUSION: This study reports a regeneration protocol from juvenile leaf explants and demonstrates that betalain stability in the stem can be maintained with 50 mM sodium ascorbate.
MATERIALS AND METHODS: In vitro shoot cultures were established on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Anatomical studies were conducted to determine the stages of shoot primordium development. Betalains were extracted from in vivo plants using 60% methanol and subjected to spectrophotometric analysis. The effect of sodium ascorbate on betalain stability was also evaluated.
RESULTS: Juvenile leaf explants regenerated shoots on MS medium supplemented with 10 μM 6-benzyladenine and 5 μM indole-3-acetic acid. Shoots were multiplied with 20 μM BA (6.25 ± 0.85 shoots/explant), and elongation was achieved with 5 μM gibberellic acid (GA3) (8.2 ± 0.37 shoots/explant). Shoot primordia developed from well-organized meristemoid cells. The betalain content in the stem was 26.66 ± 0.19 mg/100 g, but this pigment degraded within 24 h (42.19% degradation). The addition of 50 mM sodium ascorbate prevented betalain degradation, even after 24 h.
CONCLUSION: This study reports a regeneration protocol from juvenile leaf explants and demonstrates that betalain stability in the stem can be maintained with 50 mM sodium ascorbate.