[The immunological effects in guinea pig sensitized by trichloroethylene].

OBJECTIVE

To investigate the changes of certain immunological indexes in development of Guinea Pig allergic dermatitis induced by trichloroethylene (TCE).

METHODS

The Guinea Pig model of TCE-induced allergic dermatitis was established by Guinea Pig Maximisation Test. IgG levels in serum were measured by sandwich enzyme immunoassay. The splenic T lymphocytes ConA-stimulated proliferation function and natural killer (NK) cell activity were detected by MTT assay and micro-LDH release assay, respectively.

RESULTS

The sensitization rate of TCE was 66.7%. The IgG level in serum of TCE-sensitized animals was significantly higher than that in normal animals (P < 0.05). No obvious differences in Splenic T lymphocytes proliferation index and NK cell activity between TCE-sensitized and normal animals were found (P > 0.05).

CONCLUSION

Humoral immunity may play an important role in development of allergic dermatitis induced by TCE.