Bacillus safensis and Bacillus haynesii, isolated from a saline-sodium environment: Resilience to multiple stresses and tolerance to nonsteroidal anti-inflammatory drugs, based on a genomic analysis and tolerance tests.

Nonsteroidal anti-inflammatory drugs (NSAIDs) are emerging contaminants whose bioremediation becomes challenging under multiple environmental stressors. This study evaluates the resilience and NSAID tolerance of Bacillus safensis HS4-2 and Bacillus haynesii TXO1-1SG1. The two strains were isolated from saline-sodium sediment. They were tested independently for tolerance to salinity (0 - 3.4µ M), pH (5-11), and the NSAIDs diclofenac, ibuprofen, and ketoprofen (10, 100, and 1000 µM). Genetic identification was performed using the 16S rRNA gene, followed by whole-genome sequencing with the Illumina and Nanopore platforms, and annotation through RAST. The Strain HS4-2 tolerated 0 - 2.5µ M NaCl, pH 5-11, 10 µM diclofenac, 1000 µM ibuprofen, and 100 µM ketoprofen. The strain TXO1-1SG1 tolerated 0 - 2.5 µM NaCl, pH 5-11, 10 µM diclofenac, 100 µM ibuprofen, and 1000 µM ketoprofen. Both genetic and genomic analyses confirm the species assignments (Bacillus safensis HS4-2, Bacillus haynesii TXO1-1SG1). Genome annotation revealed genes associated with stress response, and aromatic compound metabolism, suggesting their potential role in NSAID transformation, although no analytical validation of degradation was performed. All sequences are available in GenBank. The dataset provides a resource for comparative and functional genomic studies of extremotolerant Bacillus strains in environments with multiple stress conditions.