[Influence of HPV infection on cell cycle regulation in epithelial cell alterations of cervix uteri: Immunohistochemical and in situ hybridization observations].

The HPV16 E6 DNA incorporation into the cervical epithelial cell genome was determined. In addition, using p16, p21 and p27 protein immunohistochemistry, we intended to present how the normal cell cycle machinery was disturbed in cervical epithelial cells. In CIN1 the epithelial cells are transformed into koilocytes, p16 is not expressed. p21 is only visible over the parabasal cell layers, while p27 manifests in koilocytes and lymphocytes. In CIN2/3, one copy of HPV16 DNA is integrated in the epithelial cell genome. In CIN3, p16 is expressed in great quantities, while p21 can be seen in the upper 2/3 segment of cervical epithelium causing minimal cell differentiation. p27 is highly expressed in the basal cells of stratified epithelium blocking the autonomous proliferation phases of the cell cycle.