Cancer treatment and research communications · 2026
"HPV 16/18 E6 DNA and mRNA expression in gross cervical lesions: A hospital based cross-sectional study from Sikkim, India".
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Lal Sunaina, Pradhan Anup, Sherpa Mingma Lhamu
Аннотация
OBJECTIVE: To assess the comparative presence of E6DNA and E6mRNA of HPV16&18 genotypes in gross cervical lesions, its correlation with cytological findings and sociodemographic factors.
METHOD: Using a cross-sectional study design, 50 women with gross cervical lesions were recruited from the gynecology clinics of the two Referral Hospitals of Sikkim. Exfoliated cervical cells were evaluated for presence of HPV16/18 E6DNA and E6mRNA using real-time PCR with an in-house designed assay. Relative expressions of E6 DNA and E6mRNA were analyzed with sociodemographic factors using logistic regression and independent sample t- test.
RESULTS: HPV16&18 E6DNA and E6mRNA was present in 46% and 50% of the samples respectively. Among women with Intraepithelial lesion, 56% (14/25) positivity was seen for HPV 16&18 E6mRNA, and 44% in women without Intraepithelial lesions. Comparatively, E6DNA was present in 40% (10/25) of women with Intraepithelial lesions and in 48% (12/25) of women without intraepithelial lesions. Younger age of sexual debut was found to be an independent predictor for HPV positivity with both DNA as well as mRNA.
CONCLUSION: Presence of HPV-E6DNA and HPV-E6mRNA differ in gross cervical lesions, Intraepithelial lesions showed higher positivity with E6mRNA as compared to E6DNA. The possible factors behind E6mRNA positivity in women with normal cytology (44%) needs further evaluation to define its clinical significance.
METHOD: Using a cross-sectional study design, 50 women with gross cervical lesions were recruited from the gynecology clinics of the two Referral Hospitals of Sikkim. Exfoliated cervical cells were evaluated for presence of HPV16/18 E6DNA and E6mRNA using real-time PCR with an in-house designed assay. Relative expressions of E6 DNA and E6mRNA were analyzed with sociodemographic factors using logistic regression and independent sample t- test.
RESULTS: HPV16&18 E6DNA and E6mRNA was present in 46% and 50% of the samples respectively. Among women with Intraepithelial lesion, 56% (14/25) positivity was seen for HPV 16&18 E6mRNA, and 44% in women without Intraepithelial lesions. Comparatively, E6DNA was present in 40% (10/25) of women with Intraepithelial lesions and in 48% (12/25) of women without intraepithelial lesions. Younger age of sexual debut was found to be an independent predictor for HPV positivity with both DNA as well as mRNA.
CONCLUSION: Presence of HPV-E6DNA and HPV-E6mRNA differ in gross cervical lesions, Intraepithelial lesions showed higher positivity with E6mRNA as compared to E6DNA. The possible factors behind E6mRNA positivity in women with normal cytology (44%) needs further evaluation to define its clinical significance.