Dietary Energy-Protein Balance Regulates Slaughter Traits, Meat Quality, Digestive Enzyme Activity, and Fecal Microbiota in Early-Weaned Gray-Feathered King Pigeon Squabs.

Early-weaned squabs are sensitive to dietary energy-protein supply, but combined effects remain unclear. We tested metabolizable energy (ME; 11.9, 12.2, and 12.5 MJ/kg) and crude protein (CP; 18, 19, and 20%) in 216 15-day-old gray-feathered King pigeon (Columba livia domestica) squabs using a 3 × 3 factorial design. Higher ME increased abdominal fat percentage, whereas CP affected slaughter traits. ME × CP interactions occurred for live weight, liver index, breast and leg muscle quality, and most digestive enzyme activities. At 18% CP, live weight was significantly higher at 12.5 than at 12.2 MJ/kg ME, whereas the opposite numerical trend occurred at 19% CP. Liver index was lower at 18% CP + 11.9 MJ/kg ME than at 18% CP + 12.2 MJ/kg ME. At 12.5 MJ/kg ME, breast muscle a* was lower at 20% CP than at 18% or 19% CP. Enzyme activities were generally highest at 20% CP + 11.9 MJ/kg ME. Targeted 16S rRNA sequencing associated the highest-energy/lowest-protein treatment with Lactobacillus-related taxa, whereas the opposite treatment showed higher diversity and greater contributions of Pseudomonadota- and Enterobacterales-related taxa. Responses were trait-specific; microbial findings represent associations between two contrasting diets.