Inoculation with autochthonous yeast strains in Harbin dry sausages with partial substitution of NaCl by KCl: Bacterial community structure and flavour profiles.

The effects of two autochthonous yeast strains (Pichia kudriavzevii and Debaryomyces hansenii) on the physicochemical characteristics, bacterial community structure, and flavour profile of the dry sausages with 40% substitution of NaCl by KCl were evaluated in this study. The results revealed that the inoculation of yeast strains increased the pH and yeast counts of low-sodium sausages. Higher contents of total esters, aldehydes, and ketones were detected in the inoculated sausages (P < 0.05). Based on the results of high-throughput sequencing, the inoculation of P. kudriavzevii decreased the abundance of Lactobacillus, Weissella, and Leuconostoc. However, the inoculation of D. hansenii increased the abundance of Lactobacillus, Weissella and Staphylococcus, which may help to inhibit the growth of pathogenic microorganisms in sausages. Electronic tongue analysis as well as sensory evaluation revealed that D. hansenii reduced bitter, astringent, and metallic tastes (P < 0.05). Overall, D. hansenii can be used as the prospective stater culture to compensate the flavour defects and improve the safety of the dry sausage with NaCl substitutes.