Frontiers in nutrition · 2026
Physicochemical characteristics and anti-colorectal cancer activity of Salvia miltiorrhiza Bunge polysaccharides.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Wang Xinran, Xu Jingjie, Liu Zhijun, Huang Kenan, Xu Fengjing, Song Xinhua, Jiao Jianpeng, Gao Shouhong, Chen Wansheng
Аннотация
INTRODUCTION: Salvia miltiorrhiza (S. miltiorrhiza) Bunge is a traditional, medicinal and edible plant with diverse biological properties.
METHODS: In this study, a novel acidic polysaccharide (SMP-3) was extracted from S. miltiorrhiza roots using hot water extraction, followed by purification through DEAE-52 cellulose and Sephadex G-100 column chromatography. Structural characterization was performed utilizing FT-IR, NMR, XRD, and CD spectroscopy. The anti-colorectal cancer activity and underlying mechanisms of SMP-3, both alone and in combination with regorafenib (REG), were evaluated in vitro using HCT116 colorectal cancer cells and normal FHC cells through cytotoxicity, Western blot, and immunofluorescence analyses.
RESULTS: The purified SMP-3 exhibited a total polysaccharide content of 96.52% with a weight-average molecular weight of 61.3 kDa. Monosaccharide composition analysis revealed that SMP-3 was primarily composed of galacturonic acid, glucuronic acid, glucose, mannose, galactose, and rhamnose in a molar ratio of 52.6:24.1:7.5:6.8:6.0:3.0, preliminarily suggesting a possible pectin-like structural feature with uronic acids accounting for 76.7% of the total content. Structural characterization indicated the presence of both α- and β-glycosidic linkages, an amorphous structure, and an ordered conformation in aqueous solution. Biologically, SMP-3 exhibited dose- and time-dependent cytotoxicity against HCT116 cells while showing minimal toxicity to normal FHC cells. Notably, combination treatment with SMP-3 and REG demonstrated combined effects, significantly enhancing G0/G1 cell cycle arrest, apoptosis induction, and inhibition of cell migration and invasion compared to single-agent treatments. Mechanistically, the combination treatment upregulated p21, Bax, and caspase-3 expression while downregulating cyclin D1, CDK4, and Bcl-2, and profoundly suppressed the phosphorylation of STAT3, AKT, and ERK signaling pathways.
DISCUSSION: These findings suggest that SMP-3 possesses distinct structural characteristics and significant anti-tumor efficacy, making it a promising medical food candidate for colorectal cancer treatment.
METHODS: In this study, a novel acidic polysaccharide (SMP-3) was extracted from S. miltiorrhiza roots using hot water extraction, followed by purification through DEAE-52 cellulose and Sephadex G-100 column chromatography. Structural characterization was performed utilizing FT-IR, NMR, XRD, and CD spectroscopy. The anti-colorectal cancer activity and underlying mechanisms of SMP-3, both alone and in combination with regorafenib (REG), were evaluated in vitro using HCT116 colorectal cancer cells and normal FHC cells through cytotoxicity, Western blot, and immunofluorescence analyses.
RESULTS: The purified SMP-3 exhibited a total polysaccharide content of 96.52% with a weight-average molecular weight of 61.3 kDa. Monosaccharide composition analysis revealed that SMP-3 was primarily composed of galacturonic acid, glucuronic acid, glucose, mannose, galactose, and rhamnose in a molar ratio of 52.6:24.1:7.5:6.8:6.0:3.0, preliminarily suggesting a possible pectin-like structural feature with uronic acids accounting for 76.7% of the total content. Structural characterization indicated the presence of both α- and β-glycosidic linkages, an amorphous structure, and an ordered conformation in aqueous solution. Biologically, SMP-3 exhibited dose- and time-dependent cytotoxicity against HCT116 cells while showing minimal toxicity to normal FHC cells. Notably, combination treatment with SMP-3 and REG demonstrated combined effects, significantly enhancing G0/G1 cell cycle arrest, apoptosis induction, and inhibition of cell migration and invasion compared to single-agent treatments. Mechanistically, the combination treatment upregulated p21, Bax, and caspase-3 expression while downregulating cyclin D1, CDK4, and Bcl-2, and profoundly suppressed the phosphorylation of STAT3, AKT, and ERK signaling pathways.
DISCUSSION: These findings suggest that SMP-3 possesses distinct structural characteristics and significant anti-tumor efficacy, making it a promising medical food candidate for colorectal cancer treatment.