Ethanol-inducible microsomal aniline hydroxylase activity and cytochrome P-450 isozymes in adult hen liver.

1. Cytochrome P-450 was induced in adult hen liver by administering 15% ethanol in drinking water and compared with other inducers such as phenobarbital and beta-naphthoflavone. 2. Aniline was the only substrate whose turnover was induced by ethanol treatment when measured in the presence of 100 microM alpha-naphthoflavone. 3. The inhibitor alpha-naphthoflavone differentiated aniline and p-nitrophenol hydroxylase activities, while p-hydroxyphenyl imidazole and SKF differentiated p-nitrophenol hydroxylase activity between ethanol- and beta-naphthoflavone-induced microsomes. 4. Ethanol treatment also slightly induced some P-450 isozymes related to phenobarbital and beta-naphthoflavone inducers.