The Science of the total environment · 2026
Wastewater-based surveillance of hepatitis A virus across municipalities and neighbourhoods in Alberta, Canada.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Weyant R Benson, Ueno Aito, Waddell Barbara, Lee Jangwoo, Bhardwaj Tulika, Acosta Nicole, Xiang Kevin, Sarabi Shahrzad, Bertazzon Stefania, Bender Aidan, Visser Gail, McCalder Janine, Papparis Chloe, Bautista Chavarriaga Maria, Clark Rhonda Gail, Fonseca Kevin, Borlang Jamie, Lee Emma, Swain Mark, Coffin Carla S, Lee Bonita, Pang Xiaoli, Cabaj Jason, O'Grady Christine, Conly John M, Hubert Casey R J, Parkins Michael D
Аннотация
BACKGROUND: Hepatitis A virus (HAV) infection is infrequently diagnosed in Canada, and little data are available regarding its epidemiology. Transmitted fecal-orally, HAV is an ideal candidate for wastewater-based surveillance (WBS). We set out to characterize HAV using WBS in a low-prevalence setting.
METHODS: This observational study was conducted in the province of Alberta, Canada. Weekly composite wastewater samples were collected from eight municipalities (populations 7909-1,306,784), and eight neighbourhoods within Calgary, the largest city. HAV genomic material was quantified using RT-qPCR targeted on VP1 and sequenced at the VP1/2A junction. HAV case data from Alberta's Public Health Laboratory and population demographics from census data were correlated with wastewater HAV burden.
RESULTS: Between July and December 2023, low levels of HAV were detected in wastewater from 50% of the municipalities and 50% of urban neighbourhoods (with 15.4% and 8.3% of samples testing positive, respectively). Wastewater HAV levels correlated with clinical HAV prevalence in larger communities. For genotyped clinical cases, wastewater with a matching VP1/2A sequence within a two-week period was observed for 8/9 episodes (median 6.42 days). Wastewater-measured HAV associated with population size, population density, and immigration from HAV endemic areas.
CONCLUSION: HAV was detectable in wastewater of both municipalities and neighbourhoods within a low prevalence setting. Wastewater HAV was associated with population metrics and socioeconomic factors, including immigration. WBS data correlated strongly with clinical disease revealing a minimal burden of undiagnosed infections. This demonstrates the feasibility of HAV WBS and its potential as a public health tool in non-endemic settings.
METHODS: This observational study was conducted in the province of Alberta, Canada. Weekly composite wastewater samples were collected from eight municipalities (populations 7909-1,306,784), and eight neighbourhoods within Calgary, the largest city. HAV genomic material was quantified using RT-qPCR targeted on VP1 and sequenced at the VP1/2A junction. HAV case data from Alberta's Public Health Laboratory and population demographics from census data were correlated with wastewater HAV burden.
RESULTS: Between July and December 2023, low levels of HAV were detected in wastewater from 50% of the municipalities and 50% of urban neighbourhoods (with 15.4% and 8.3% of samples testing positive, respectively). Wastewater HAV levels correlated with clinical HAV prevalence in larger communities. For genotyped clinical cases, wastewater with a matching VP1/2A sequence within a two-week period was observed for 8/9 episodes (median 6.42 days). Wastewater-measured HAV associated with population size, population density, and immigration from HAV endemic areas.
CONCLUSION: HAV was detectable in wastewater of both municipalities and neighbourhoods within a low prevalence setting. Wastewater HAV was associated with population metrics and socioeconomic factors, including immigration. WBS data correlated strongly with clinical disease revealing a minimal burden of undiagnosed infections. This demonstrates the feasibility of HAV WBS and its potential as a public health tool in non-endemic settings.