Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2011
[The regulation of T3 on maldeveloped gonocyte (Go) and expression of NCAM in flutamide-induced cryptorchidism rat].
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Ma Chao, He Da-Wei, Jiang Yong-Chao, Zhao Dan, He Wen-Fei, Wei Guang-Hui
Аннотация
OBJECTIVE: To investigate the regulation of T3 on the maldeveloped gonocytes (Go) and the expression of NCAM in Flutamide-induced cryptorchidism SD rat.
METHODS: Postnatal day (PD) 1 and PD20 cryptorchidism SD rat induced by Flu were treated with 15 microg/(kg x d) T3 by subcutaneous injections. Incidence of the cryptorchidism, histology were evaluated. And the expression of NCAM was detected by immunohistochemisty and RT-PCR.
RESULTS: The incidence of cryptorchidism in the group of Flu-induced offsprings treated by 15 microg/(kg x d) T3 from PD1 was 25% (8/32), which was lower than that of Flu group 40.9% (9/22). No Go and NCAM expression were observed in the seminiferous tubules. NCAM mRNA expression in Flu-induced PD13, PD20 testes was up-regulated when compared with control (P < 0.01, P < 0.05), while after treated with 15 microg/ (kg x d) T3 the NCAM expression was significantly down-regulated compared to that of non T3 treated group (P < 0.01), and was not significantly different from that of control (P > 0.05).
CONCLUSION: The incidence of Flu-induced cryptorchidism can be decreased by T3 treatment after birth. T3 may contribute to the down-regulation of the NCAM expression, and reduction or elimination of the remainded gonocytes in crytorcidism seminiferous tubules.
METHODS: Postnatal day (PD) 1 and PD20 cryptorchidism SD rat induced by Flu were treated with 15 microg/(kg x d) T3 by subcutaneous injections. Incidence of the cryptorchidism, histology were evaluated. And the expression of NCAM was detected by immunohistochemisty and RT-PCR.
RESULTS: The incidence of cryptorchidism in the group of Flu-induced offsprings treated by 15 microg/(kg x d) T3 from PD1 was 25% (8/32), which was lower than that of Flu group 40.9% (9/22). No Go and NCAM expression were observed in the seminiferous tubules. NCAM mRNA expression in Flu-induced PD13, PD20 testes was up-regulated when compared with control (P < 0.01, P < 0.05), while after treated with 15 microg/ (kg x d) T3 the NCAM expression was significantly down-regulated compared to that of non T3 treated group (P < 0.01), and was not significantly different from that of control (P > 0.05).
CONCLUSION: The incidence of Flu-induced cryptorchidism can be decreased by T3 treatment after birth. T3 may contribute to the down-regulation of the NCAM expression, and reduction or elimination of the remainded gonocytes in crytorcidism seminiferous tubules.