Simultaneous determination of multiple endocrine disrupting chemicals in human amniotic fluid samples by solid phase extraction coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS).

Prenatal exposure to endocrine-disrupting chemicals (EDCs) may lead to immediate and long-term adverse health effects in the offspring. The occurrence levels of EDCs in amniotic fluid (AF) have significant influence on fetus prior to birth. In this study, an analytical method for simultaneous determination of a total of 59 widely concerned EDCs, including 11 bisphenols (BPs), 4 parabens, 11 phthalate metabolites (mPAEs), 7 organophosphate metabolites (mOPEs), 8 polycyclic aromatic hydrocarbons metabolites (OH-PAHs) and 18 perfluorinated compounds (PFASs), in human AF samples was developed employing solid phase extraction (SPE) coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS). Key parameters of SPE procedures were carefully investigated. The developed method enabled the determination of 59 EDCs in a low sample volume (500 μL) with LOD and LOQ values in the range of 0.02-0.2 ng/mL and 0.07-0.66 ng/mL, respectively. The recoveries (60.95 %-144.74 %) and precisions (<44 %) were obtained. To further verify the method, AF samples from Tianjin of China were collected and analyzed. The detected concentrations of BPs, parabens, mPAEs, mOsPEs, and PFASs ranged from < LOD-60.48 ng/mL, indicating satisfying performance for field samples. Furthermore, the results of AGREEprep, ComplexGAPI and BAGI exhibited good environmental friendliness and applicability of the developed method.