Arerugi = [Allergy] · 2010
[Effect of osmolality on ion transport in Guinea pig airway epithelial cells].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Hattori Tomohiro, Majima Toru, Hashimoto Shu
Аннотация
BACKGROUND: Increased osmolality of the airway surface fluid due to water loss associated with hyperventilation is considered to be a cause of exercise-induced asthma (EIA).
AIM: We investigated the influence of changes in osmolality on airway epithelial ion transport in guinea pigs.
METHODS: We explored from measuring open circuit potential difference in challenge with hypertonic saline or mannitol.
RESULTS: Diphenylamine-2-carboxylate (DPC), amiloride, and disodium cromoglycate (DSCG) prevented an increase of the potential difference (PD) after exposure of tracheal mucosa to 0.9-10.8% hypertonic saline solutions (HSSs) (p < 0.05, p < 0.01, and p < 0.01, respectively on 1.8%). An increase of the PD was observed after a single dose of 1.8% hypertonic saline solution (HSS) was applied to the mucosa, but no increase was observed after a single exposure to a 585 mOsm/kg aqueous mannitol solution (585AMS). The results remained the same when a Cl-free solution was used as the perfusate. The change of airway epithelial cell thickness was only suppressed significantly by DPC (p < 0.01) after 1.8% HSS challenge.
CONCLUSIONS: Not only the change of osmolality, but also changes of the Cl- and Na+ concentrations in airway surface fluid seem to have an important influence on the PD. cAMP-dependent Cl- channel may have a role, and the same mechanism may provoke EIA.
AIM: We investigated the influence of changes in osmolality on airway epithelial ion transport in guinea pigs.
METHODS: We explored from measuring open circuit potential difference in challenge with hypertonic saline or mannitol.
RESULTS: Diphenylamine-2-carboxylate (DPC), amiloride, and disodium cromoglycate (DSCG) prevented an increase of the potential difference (PD) after exposure of tracheal mucosa to 0.9-10.8% hypertonic saline solutions (HSSs) (p < 0.05, p < 0.01, and p < 0.01, respectively on 1.8%). An increase of the PD was observed after a single dose of 1.8% hypertonic saline solution (HSS) was applied to the mucosa, but no increase was observed after a single exposure to a 585 mOsm/kg aqueous mannitol solution (585AMS). The results remained the same when a Cl-free solution was used as the perfusate. The change of airway epithelial cell thickness was only suppressed significantly by DPC (p < 0.01) after 1.8% HSS challenge.
CONCLUSIONS: Not only the change of osmolality, but also changes of the Cl- and Na+ concentrations in airway surface fluid seem to have an important influence on the PD. cAMP-dependent Cl- channel may have a role, and the same mechanism may provoke EIA.