Herpes Simplex Virus Type 1 and Type 2 Western Blot Serology Test Results From 1999 to 2020 in a US Reference Laboratory.

We analyzed 162,397 deidentified HSV Western blot results performed at the University of Washington Virology Laboratory from 1999 to 2020. The seroprevalence of HSV-1 declined by 0.53% per year, whereas indeterminate HSV-1 results increased by 0.08% annually. Similarly, HSV-2 seroprevalence decreased by 0.72%, and indeterminate results increased by 0.16% each year.