Nature communications · 2023
A natural mutation in the promoter of Ms-cd1 causes dominant male sterility in Brassica oleracea.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Han Fengqing, Yuan Kaiwen, Sun Wenru, Zhang Xiaoli, Liu Xing, Zhao Xinyu, Yang Limei, Wang Yong, Ji Jialei, Liu Yumei, Li Zhansheng, Zhang Jinzhe, Zhang Chunzhi, Huang Sanwen, Zhang Yangyong, Fang Zhiyuan, Lv Honghao
Аннотация
Male sterility has been used for crop hybrid breeding for a long time. It has contributed greatly to crop yield increase. However, the genetic basis of male sterility has not been fully elucidated. Here, we report map-based cloning of the cabbage (Brassica oleracea) dominant male-sterile gene Ms-cd1 and reveal that it encodes a PHD-finger motif transcription factor. A natural allele Ms-cd1PΔ-597, resulting from a 1-bp deletion in the promoter, confers dominant genic male sterility (DGMS), whereas loss-of-function ms-cd1 mutant shows recessive male sterility. We also show that the ethylene response factor BoERF1L represses the expression of Ms-cd1 by directly binding to its promoter; however, the 1-bp deletion in Ms-cd1PΔ-597 affects the binding. Furthermore, ectopic expression of Ms-cd1PΔ-597 confers DGMS in both dicotyledonous and monocotyledonous plant species. We thus propose that the DGMS system could be useful for breeding hybrids of multiple crop species.