International journal of medical microbiology : IJMM · 2025
Virulome and genome-wide association study of vancomycin-resistant Enterococcus faecium from bloodstream infections versus colonization isolates.
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Böing Christian W, Schneider Julia S, Froböse Neele J, Jurke Annette, Kampmeier Stefanie, Mellmann Alexander
Аннотация
INTRODUCTION: Vancomycin-resistant Enterococcus faecium (VREfm) are multidrug-resistant pathogens that cause severe nosocomial infections. The knowledge of virulence profiles of VREfm for the development of invasive infections is still limited. The aim of this study was to analyse gene profiles and genetic variants of VREfm and their association with the development of VREfm bloodstream infections (VRE-BSI).
METHODS: One-hundred twenty randomly selected VRE-BSI isolates from a state-wide surveillance study in the German state of North Rhine-Westphalia were included in the analysis and matched with one-hundred twenty VREfm colonization (VRE-COL) isolates from a tertiary hospital in North Rhine-Westphalia. All isolates were subjected to whole genome sequencing (WGS) and analysed for the presence or absence of known or putative virulence genes of E. faecium. A genome-wide association study (GWAS) approach was conducted to identify potential associations of genes and genetic variants with the development of VRE-BSI.
RESULTS: The multilocus sequence typing (MLST) sequence types (ST) ST80 and ST117 were the most prevalent STs among VRE-BSI and VRE-COL isolates (ST80: 133 [55 %]; ST117: 98 [41 %]). ST80 was significantly more prevalent in VRE-BSI isolates compared to VRE-COL isolates (75 [63 %] vs. 58 [48 %], p = 0.027). Only fms21 (pilA), a gene of pili gene cluster 1 (PCG-1), coding for a cell-wall-anchored protein involved in adhesive processes, was significantly more prevalent in the VRE-BSI group. GWAS identified no associations of genes or variants with VRE-BSI.
CONCLUSION: Using comparative genomics and GWAS, we could not identify a distinct virulence profile for the development of VRE-BSI.
METHODS: One-hundred twenty randomly selected VRE-BSI isolates from a state-wide surveillance study in the German state of North Rhine-Westphalia were included in the analysis and matched with one-hundred twenty VREfm colonization (VRE-COL) isolates from a tertiary hospital in North Rhine-Westphalia. All isolates were subjected to whole genome sequencing (WGS) and analysed for the presence or absence of known or putative virulence genes of E. faecium. A genome-wide association study (GWAS) approach was conducted to identify potential associations of genes and genetic variants with the development of VRE-BSI.
RESULTS: The multilocus sequence typing (MLST) sequence types (ST) ST80 and ST117 were the most prevalent STs among VRE-BSI and VRE-COL isolates (ST80: 133 [55 %]; ST117: 98 [41 %]). ST80 was significantly more prevalent in VRE-BSI isolates compared to VRE-COL isolates (75 [63 %] vs. 58 [48 %], p = 0.027). Only fms21 (pilA), a gene of pili gene cluster 1 (PCG-1), coding for a cell-wall-anchored protein involved in adhesive processes, was significantly more prevalent in the VRE-BSI group. GWAS identified no associations of genes or variants with VRE-BSI.
CONCLUSION: Using comparative genomics and GWAS, we could not identify a distinct virulence profile for the development of VRE-BSI.