Lectin histochemistry of bone regeneration dynamics in the rabbit mandible following transplantation of natural mineral and organic osteoplastic materials.

OBJECTIVE

Aim: To reveal the applicability of lectin histochemistry methods for the evaluation of bone tissue injury recovery.

PATIENTS AND

METHODS

Materials and

Methods

The research was accomplished on 35 mature rabbits aged 6-7 months weighting 2.5-3 kg subdivided into three groups. Control group included 10 rabbits with post traumatic mandibular bone injury site healed under the blood cloth. First experimental group consisted of animals with damaged bone recovery supplemented with an osteotropic material based on native octacalcium phosphate (OCP-N). Second experimental group consisted of rabbits with bone defect filled with natural collagen cone (Col-C). Monitoring of the post-traumatic processing. was carried out for 84 days using panel of three lectins (WGA, LABA and CNFA) with different carbohydrate specificities.

RESULTS

Results

The lectins demonstrated high variability of binding with constituents of posttraumatic bone tissue. In particular, it was revealed rather selective WGA reactivity with microvascular endothelium, LABA reactivity with macrophages and CNFA with fibroblaststs and osteogenic cells. Injured bone in control and both experimental groups demonstrated distinct rearrangement of lectin receptor sites with drift towards posttraumatic recovery being more prominent after aplication of OCP-N osteotropic material. Traumatic bone injury induced accumulation of macrophages in the site of inflammation, as well as redistribution of lectin receptors in microvascular endothelium.

CONCLUSION

Conclusions

Lectins of different carbohydrate specificities can be recommended for monitoring bone tissue regeneration after posttraumatic injuries.