In Vitro Assessment of Stimulation of Collagen Biosynthesis via Collagenprash and Collagen Builder.

The skin serves as the primary physical barrier and plays a critical role in the aesthetic appearance, particularly through its pigmentation and texture. Collagenprash and Collagen builder are developed to support skin integrity by promoting collagen-associated protein expression. This study systematically evaluated these claims through a variety of biochemical and cellular assays. The formulations were assessed for biochemical and cellular antioxidant activity, collagenase inhibition, interleukin-mediated anti-inflammatory activity, melanogenesis inhibition, and proteins associated with collagen expression by using immunofluorescence analysis. The presence of a collagen blend, ascorbic acid, and botanical extracts enhanced the expression of prolyl 4-hydroxylase (P4H) and lysyl hydroxylase, key enzymes involved in post-translational modification of collagen. Upregulation of P4H facilitates hydroxylation of proline residues, a critical step for stabilization of the collagen triple helix, whereas increased lysyl hydroxylase expression promotes hydroxylysine formation and subsequent intermolecular cross-linking. Therefore, these formulations may have the potential to support collagen biosynthesis and maintain skin texture.