China CDC weekly · 2025
Evaluation of Measles Vaccine Immunogenicity and Durability Using A Pseudotyped Virus Neutralization Assay.
СтатьяНазвание и аннотация: Перевод в очереди
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Jiang Qi, Wu Xi, Zhang Jie, Wang Huiling, Dong Fangyu, Wu Xuelian, Yu Pengju, Nie Jianhui, Wang Youchun, Huang Weijin, Zhou Jiuyue, Quan Yaru, Zhang Yan, Wang Suting, Li Juan
Аннотация
INTRODUCTION: This study aimed to establish a robust method for monitoring measles vaccine-induced immunity and assessing population-level serostatus.
METHODS: This study constructed a vesicular stomatitis virus (VSV)-based pseudotyped virus system expressing envelope proteins from seven major circulating measles genotypes (H1, B3, D4, D8, D9, D11, G3) and the Schwarz vaccine strain (genotype A), thereby enabling a high-throughput neutralization assay for antibody detection.
RESULTS: Vaccination induced a substantial increase in neutralizing antibody geometric mean titers (GMT) post-immunization (4,808 after the first dose; 5,326 after the second dose), with antibody levels remaining elevated in 4-year-old children (GMT: 3,834). Cross-neutralization activity against different genotypes varied by less than 6.4-fold, demonstrating broad protective immunity. However, 12% of adult sera tested were seronegative, revealing the presence of susceptible populations.
CONCLUSIONS: This study confirms the robust immunogenicity of the current measles vaccine and establishes a valuable tool for serosurveillance and long-term immunity assessment.
METHODS: This study constructed a vesicular stomatitis virus (VSV)-based pseudotyped virus system expressing envelope proteins from seven major circulating measles genotypes (H1, B3, D4, D8, D9, D11, G3) and the Schwarz vaccine strain (genotype A), thereby enabling a high-throughput neutralization assay for antibody detection.
RESULTS: Vaccination induced a substantial increase in neutralizing antibody geometric mean titers (GMT) post-immunization (4,808 after the first dose; 5,326 after the second dose), with antibody levels remaining elevated in 4-year-old children (GMT: 3,834). Cross-neutralization activity against different genotypes varied by less than 6.4-fold, demonstrating broad protective immunity. However, 12% of adult sera tested were seronegative, revealing the presence of susceptible populations.
CONCLUSIONS: This study confirms the robust immunogenicity of the current measles vaccine and establishes a valuable tool for serosurveillance and long-term immunity assessment.