Proteomics reveals plasma profiles for monitoring the toxicity caused by chromium compounds.

BACKGROUND

Today, various heavy metals are widespread in the ecosystem and have become important environmental contaminants. Exposure to these hazardous metals such as chromium usually results in cytotoxicity and large-scale protein changes which reflect pathologic states.

METHODS

We used a comprehensive proteomic tool to survey changes in plasma proteins elicited by two chromium species (Cr(6+) and Cr(3+)). RT-PCR was applied to evaluate levels of cytokines associated with adverse responses. Lectin blotting was used to investigate the contents of fucosylated proteins.

RESULTS

Protein profiles revealed statistically significant changes in the intensity of 12 proteins. The network analysis implied that Cr(6+) application strongly induced the IL-6-stimulated inflammatory pathway. mRNA levels of specific cytokines were also correlated with inflammatory events. Increased IL-6 modulation of the fucosylation of haptoglobin was also identified in Cr(6+)-treated samples.

CONCLUSIONS

These results suggest that Cr(6+) may induce IL-6-mediated inflammatory responses which result in hepatic injury. This paper highlights the applications of functional proteomics of plasma profiles and fucosylated glycoproteins as a predictive tool to monitor human health in contact with chromium.