Gaseous signaling molecule H2S as a multitasking signal molecule in ROS metabolism of Oryza sativa under thiocyanate (SCN-) pollution.

The induction of disruption in the electronic transport chain by thiocyanate (SCN-) leads to an excessive generation of reactive oxygen species (ROS) within rice (Oryza sativa). Hydrogen sulfide (H2S) assumes a crucial role as a gaseous signaling molecule, holding significant potential in alleviating SCN--related stress. Nevertheless, there remains a dearth of understanding regarding the intricate interplay between H2S and ROS in Oryza sativa amidst SCN- pollution. In this investigation, a hydroponics-based experiment was meticulously devised to explore how H2S-mediated modifications influence the genetic feedback network governing ROS metabolism within the subcellular organelles of Oryza sativa when exposed to varying effective concentrations (EC20: 24 mg SCN/L; EC50: 96 mg SCN/L; EC75: 300 mg SCN/L) of SCN-. The findings unveiled the enhanced capacity of Oryza sativa to uptake SCN- under H2S + SCN- treatments in comparison to SCN- treatments alone. Notably, the relative growth rate (RGR) of seedlings subjected to H2S + SCN- exhibited a superior performance when contrasted with seedlings exposed solely to SCN-. Furthermore, the application of exogenous H2S yielded a significant reduction in ROS levels within Oryza sativa tissues during SCN- exposure. To elucidate the intricacies of gene regulation governing ROS metabolism at the mRNA level, the 52 targeted genes were categorized into four distinct types, namely: initial regulatory ROS generation genes (ROS-I), direct ROS scavenging genes (ROS-II), indirect ROS scavenging genes (ROS-III), and lipid oxidation genes (ROS-IV). On the whole, exogenous H2S exhibited the capacity to activate the majority of ROS-I ∼ ROS-IV genes within both Oryza sativa tissues at the EC20 concentration of SCN-. However, genetic positive/negative feedback networks emphasized the pivotal role of ROS-II genes in governing ROS metabolism within Oryza sativa. Notably, these genes were predominantly activated within the cytoplasm, chloroplasts, mitochondria, peroxisomes, and the cell wall.