Prevalence and characterization of bacterial rectal colonization patterns in pediatric patients: A cross-sectional study.

BACKGROUND

Antimicrobial resistance is a major public health concern requiring ongoing surveillance to determine its extent and associated factors. This study evaluates bacterial isolates from pediatric patients in terms of distribution, resistance mechanisms, and demographic/clinical correlations.

METHODS

In this cross-sectional study, after obtaining parental assent, rectal swabs were aseptically collected from children by pediatric doctors at Healthcare Centers in Beirut. Necessary data were collected through validated questionnaires provided to parents. Bacterial identification was performed using standard culture, isolation, and biochemical techniques. Susceptibility of bacterial strains was studied using antimicrobial susceptibility testing. Finally, Polymerase Chain Reaction (PCR) was used to characterize resistance genes. Associations between resistance phenotypes and sample characteristics were assessed using chi-square or Fisher's exact test, as appropriate.

RESULTS

Among 118 rectal isolates, Escherichia coli predominated (75.4 %), followed by other Enterobacterales and Staphylococci. Overall, 31.36 % of isolates harbored a major resistance mechanism (ESBL, AmpC, CRE, or MRSA), with ESBL-producing E. coli being the most frequent (22 %) and blaCTX-M detected in the majority of ESBL isolates. Previous antacid intake was the only characteristic significantly associated with resistance phenotypes (p = 0.03). AmpC, CRE, and MRSA remained relatively infrequent but represented clinically important resistance mechanisms.

CONCLUSION

Rectal colonization with multidrug-resistant organisms was frequent, driven mainly by ESBL-producing, blaCTX-M-positive E. coli, while AmpC, CRE, and MRSA were less common but remained important resistance mechanisms. The association between resistance phenotypes and prior antacid use highlights the need for larger pediatric surveillance studies and careful evaluation of antacid and antibiotic use in this population.