[Effect of methyl viologen on the Ca(2+)-ATPase activities of membrane vesicles in roots of rice seedlings].

The cold tolerance of rice (Oryza sativa L.) seedlings could be markedly improved by mild oxidative pretreatment (10 mumol/L methyl viologen (MV). The enhancement of chilling resistance induced by MV pretreatment could be inhibited by Ca(2+)-chelate ethyleneglycol-bis-(beta-aminoethylether) N,N-tetraacetic acid (EGTA, 10 mmol/L) and the calmodulin inhibitor chlorpromazine (CPZ, 0.5 mmol/L). The Ca(2+)-ATPase activity of root plasmolemma and tonoplast membrane as well as the Fe(CN)6(3-) reduction of plasmolemma were also enhanced by MV pretreatment. However, the above MV pretreatment effect could be inhibited by EGTA and CPZ, respectively. In vitro, Ca(2+)-ATPase activities in the two types of membrane vesicles were reduced by reactive oxygen species (H2O2, O2-., .OH). Results indicated that the enhancement of chilling resistance in rice seedlings with MV pretreatment was likely related to the effective activation of Ca(2+)-ATPase induced by calcium messenger of CaM, and the improved cold tolerance of the activities might be associated with the augmentation of cellular membrane protective ability caused by the MV pretreatment.