Zhonghua fu chan ke za zhi · 1998
[Apoptosis of human oocytes is age-related].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Wu J, Zhang L
Аннотация
OBJECTIVE: To study the morphological characteristics, and rate of apoptosis of human oocytes in relation to age.
METHODS: 206 oocytes were aspirated from antral follicles 4-6 mm in diameter from 58 patients who had surgery done for ovarian cysts. These oocytes were incubated in Ham's F10 with 10% fetal cord serum (FCS) for 48-120 hours, and examined under inverted microscope every 6-8 hours to see their morphological changes. In situ end-labeling (ISEL) of fragmented DNA was used to confirm apoptosis.
RESULTS: The characteristics of apoptosis are: (1) shape change of oocytes. (2) oocyte shrinkage. (3) chromatic condensation and apoptotic body formation. (4) internucleosomal DNA cleavage was shown by ISEL. Apoptosis occurred in 13.9% (women aged 21-30), 37.7% (women aged 31-40), and 61.5% (women aged 41-50) respectively. The rate of apoptosis was much higher in women aged 41-50 than that in the other two groups.
CONCLUSION: The rate of apoptosis of human immature oocytes cultured in vitro is much higher in older women aged 41-50 than in those aged 21-40, which may account for the poor response to superovulation stimulation and low oocyte quality in older women.
METHODS: 206 oocytes were aspirated from antral follicles 4-6 mm in diameter from 58 patients who had surgery done for ovarian cysts. These oocytes were incubated in Ham's F10 with 10% fetal cord serum (FCS) for 48-120 hours, and examined under inverted microscope every 6-8 hours to see their morphological changes. In situ end-labeling (ISEL) of fragmented DNA was used to confirm apoptosis.
RESULTS: The characteristics of apoptosis are: (1) shape change of oocytes. (2) oocyte shrinkage. (3) chromatic condensation and apoptotic body formation. (4) internucleosomal DNA cleavage was shown by ISEL. Apoptosis occurred in 13.9% (women aged 21-30), 37.7% (women aged 31-40), and 61.5% (women aged 41-50) respectively. The rate of apoptosis was much higher in women aged 41-50 than that in the other two groups.
CONCLUSION: The rate of apoptosis of human immature oocytes cultured in vitro is much higher in older women aged 41-50 than in those aged 21-40, which may account for the poor response to superovulation stimulation and low oocyte quality in older women.