[Effects of fluid shear stress time on carbonic anhydrase in rat osteoclasts].

PURPOSE: To observe the effects of fluid shear stress on carbonic anhydrase II in rat osteoclasts.

METHODS

Osteoclasts were subject to fluid shear stress for 0,15,30,60 and 120 minutes respectively, which was 2.9dyne/cm(2). mRNA level of carbonic anhydrase II was detected through real-time fluorescent quantitation PCR. The data were analyzed with SPSS 12.0 software package.

RESULTS

mRNA level of carbonic anhydrase II in control group, 15 minutes,30 minutes,60 minutes and 120 minutes group was 7.88+/-0.09, 11.14+/-0.12, 15.83+/-0.18, 1.94+/-0.02, and 1.37+/-0.01(E+5 copy number), respectively. There was significant difference between any two groups (P<0.05).

CONCLUSIONS

In this present study, mRNA level of carbonic anhydrase II has an increasing tendency with various action times of fluid shear stress, it reaches to a peak value at 30 minutes. We speculated that there might be a certain time-effect relation between fluid shear stress and mRNA level of carbonic anhydrase II, but this mechanism need be further studied.