Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2009
[Effects of natural plant ginkgolic acids on the apoptosis of human Hep-2 cancer cells].
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Zhou Chen-chen, Du Wei, Wen Zong, Li Ji-yao, Zhang Ping
Аннотация
OBJECTIVE: To investigate the anti-cancer effects and mechanism of natural plant ginkgolic acids (GAs) on Human Hep-2 cancer cells.
METHODS: Hep-2 cancer cells were treated with different concentration of GAs for different times. The proliferation of Hep-2 cancer cells were detected by MTT colorimetric assay. Biochemistry characteristics of cell apoptosis was analyzed by DNA agarose electrophoresis and FCM.
RESULTS: The proliferation of Hep-2 cancer cells were inhibited significantly by GAs in a dose and time-dependent manner. Typical DNA ladder bands of Hep-2 cell DNA treated with GAs were observed on agarose electrophoresis gels. Increased apoptosis of Hep-2 cells treated with GAs was also noticed with FCM analysis.
CONCLUSION: Ginkgolic acids show an anti-tumor activity through inhibition the growth and inducing apoptosis of tumor cell in vitro.
METHODS: Hep-2 cancer cells were treated with different concentration of GAs for different times. The proliferation of Hep-2 cancer cells were detected by MTT colorimetric assay. Biochemistry characteristics of cell apoptosis was analyzed by DNA agarose electrophoresis and FCM.
RESULTS: The proliferation of Hep-2 cancer cells were inhibited significantly by GAs in a dose and time-dependent manner. Typical DNA ladder bands of Hep-2 cell DNA treated with GAs were observed on agarose electrophoresis gels. Increased apoptosis of Hep-2 cells treated with GAs was also noticed with FCM analysis.
CONCLUSION: Ginkgolic acids show an anti-tumor activity through inhibition the growth and inducing apoptosis of tumor cell in vitro.