Ultrasound in medicine & biology · 2025
Quantitative Contrast-Enhanced Ultrasound Assessment of Renal Microcirculation and Function in a Rat Model of Sepsis-Induced Acute Kidney Injury.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Liu Peiqing, Cai Xiaowei, Chen Huijuan, Li Yecheng, Zhang Ying, Ding Changwei, Hou Yan, Zhang Yingchun
Аннотация
PURPOSE: This study aims to investigate the utility of quantitative contrast-enhanced ultrasound (CEUS) in both assessing renal perfusion and monitoring renal function in a rat model of sepsis-associated acute kidney injury (S-AKI).
METHODS: Thirty-six healthy Sprague-Dawley rats were divided into a sepsis model group (with severe and mild subgroups) and a sham-operated control group (Sham). The model subgroups underwent cecal ligation and puncture to induce S-AKI, while the Sham group received only a laparotomy without ligation or puncture. Jugular venous blood was collected to measure serum creatinine (Scr) and uric acid (UA) levels. CEUS was performed to evaluate renal perfusion and time-intensity curves were analyzed to derive perfusion parameters. The relationships between these CEUS-derived parameters and the serum biomarkers were examined. Finally, kidney tissue samples were harvested for pathological analysis to confirm AKI.
RESULTS: In the sepsis model rats, the time-based perfusion parameters-time to peak (TTP), rise time (RT), fall half-time (FHT) and mean transit time (MTT)-were all significantly longer than in the Sham group (all p < 0.05). Scr and UA levels correlated positively with TTP, MTT, RT and FHT (Scr: r = 0.695, 0.669, 0.704, 0.394; UA: r = 0.810, 0.798, 0.586, 0.351; all p < 0.05). Histopathological examination of renal tissues in the model subgroups revealed changes consistent with AKI, with the severe subgroup exhibiting more pronounced renal damage than the mild subgroup.
CONCLUSION: Quantitative CEUS can effectively evaluate renal perfusion in rats with S-AKI and is valuable for detecting and monitoring changes in renal function.
METHODS: Thirty-six healthy Sprague-Dawley rats were divided into a sepsis model group (with severe and mild subgroups) and a sham-operated control group (Sham). The model subgroups underwent cecal ligation and puncture to induce S-AKI, while the Sham group received only a laparotomy without ligation or puncture. Jugular venous blood was collected to measure serum creatinine (Scr) and uric acid (UA) levels. CEUS was performed to evaluate renal perfusion and time-intensity curves were analyzed to derive perfusion parameters. The relationships between these CEUS-derived parameters and the serum biomarkers were examined. Finally, kidney tissue samples were harvested for pathological analysis to confirm AKI.
RESULTS: In the sepsis model rats, the time-based perfusion parameters-time to peak (TTP), rise time (RT), fall half-time (FHT) and mean transit time (MTT)-were all significantly longer than in the Sham group (all p < 0.05). Scr and UA levels correlated positively with TTP, MTT, RT and FHT (Scr: r = 0.695, 0.669, 0.704, 0.394; UA: r = 0.810, 0.798, 0.586, 0.351; all p < 0.05). Histopathological examination of renal tissues in the model subgroups revealed changes consistent with AKI, with the severe subgroup exhibiting more pronounced renal damage than the mild subgroup.
CONCLUSION: Quantitative CEUS can effectively evaluate renal perfusion in rats with S-AKI and is valuable for detecting and monitoring changes in renal function.