Clinical value and pro-inflammatory mechanism of miR-503-5p as a novel diagnostic biomarker for Sepsis.

AIMS: Explore the expression of miR-503-5p in sepsis and its influence on the inflammatory response.

METHODS

A total of 120 patients with sepsis were retrospectively selected as the research subjects, and 80 healthy individuals were simultaneously selected as the control group. Reverse transcription quantitative polymerase chain reaction was used to detect the expression of miR-503-5p and related genes. Pearson correlation analysis was used to determine the relationships between variables, and multivariate logistic regression analysis was applied to analyze the risk factors associated with sepsis. Receiver operating characteristic curve analysis was performed to assess the diagnostic efficacy of serum miR-503-5p levels for sepsis. Sepsis-model cells were created in vitro for the purpose of exploring the possible action mechanism of miR-503-5p in the context of sepsis.

RESULTS

The expression of miR-503-5p in the serum of patients with sepsis is significantly upregulated, and it is positively correlated with the severity of the patients' condition and inflammatory indexes. miR-503-5p has a good diagnostic potential in sepsis. Furthermore, miR-503-5p exhibited a notable increase in expression within lipopolysaccharide (LPS)-stimulated THP-1 cells, which facilitated the inflammatory process. Mechanistically, miR-503-5p exacerbates the inflammatory response in sepsis via targeted suppression of peroxisome proliferator-activated receptor alpha (PPARA).

CONCLUSIONS

During sepsis, miR-503-5p shows elevated expression, and it facilitates the inflammatory response through the targeted suppression of PPARA.