Epitranscriptome Mapping of N6-Methyladenosine Using m6A Immunoprecipitation with High Throughput Sequencing in Skeletal Muscle Stem Cells.

N6-Methyladenosine (m6A), one of the most abundant chemical modifications in mRNA (epitranscriptome), contributes to the regulation of biological processes by iterating gene expression post-transcriptionally. A number of publications on m6A modification have escalated in the recent past, due to the advancements in profiling m6A along the transcriptome using different approaches. The vast majority of studies primarily focused on m6A modification on cell lines but not primary cells. We present in this chapter a protocol for m6A immunoprecipitation with high throughput sequencing (MeRIP-Seq) that profiles m6A on mRNA with merely 100 μg total RNA worth of muscle stem cells as starting material. With this MeRIP-Seq, we observed epitranscriptome landscape in muscle stem cells.