Pakistan journal of biological sciences : PJBS · 2020
Evaluation of the Antibacterial Activity of Spathiphyllum wallisii Extracts Against Human Pathogenic Bacteria.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Rattanasuk Surachai, Phiwthong Tannatorn
Аннотация
BACKGROUND AND OBJECTIVE: The urgent of finding new antibiotics due to the rising of antibiotic-resistant bacteria. The plant is the main source of new antibiotic substances. The purpose of this research was to evaluate the antibacterial activity of Spathiphyllum wallisii extracts against nine human pathogenic bacteria.
MATERIALS AND METHODS: The stalks, leaf, rhizome and root of S. wallisii were extracted by using hexane, dichloromethane, ethyl acetate, ethanol and methanol. The disc diffusion assay was used to screen the antibacterial activity of S. wallisii extracts. Broth dilution and colorimetric assay were used to determine the Minimal inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) values of extracts.
RESULTS: The lowest MIC values at 0.048 mg mL-1 were presented in the stalks extract with dichloromethane, ethyl acetate, methanol and ethanol against B. subtilis TISTR 008, the leaf extracted with hexane, dichloromethane, ethyl acetate, methanol and ethanol against B. subtilis TISTR 008; the leaf extracted with ethyl acetate, methanol and ethanol against S. aureus TISTR 1466, the leaf extracted with dichloromethane, ethyl acetate, methanol and ethanol against S. aureus PK; the rhizome extracted with methanol against S. aureus PK. The lowest of MBC value of 0.048 mg mL-1 was obtained from methanolic rhizome extract against B. subtilis TISTR 008.
CONCLUSION: The methanolic rhizome extract of S. wallisii demonstrated the highest of pathogenic bacterial growth inhibition. This is the first report about the antibacterial activity of S. wallisii extracts that will add new information in natural drug discovery and development in industrial pharmacology.
MATERIALS AND METHODS: The stalks, leaf, rhizome and root of S. wallisii were extracted by using hexane, dichloromethane, ethyl acetate, ethanol and methanol. The disc diffusion assay was used to screen the antibacterial activity of S. wallisii extracts. Broth dilution and colorimetric assay were used to determine the Minimal inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) values of extracts.
RESULTS: The lowest MIC values at 0.048 mg mL-1 were presented in the stalks extract with dichloromethane, ethyl acetate, methanol and ethanol against B. subtilis TISTR 008, the leaf extracted with hexane, dichloromethane, ethyl acetate, methanol and ethanol against B. subtilis TISTR 008; the leaf extracted with ethyl acetate, methanol and ethanol against S. aureus TISTR 1466, the leaf extracted with dichloromethane, ethyl acetate, methanol and ethanol against S. aureus PK; the rhizome extracted with methanol against S. aureus PK. The lowest of MBC value of 0.048 mg mL-1 was obtained from methanolic rhizome extract against B. subtilis TISTR 008.
CONCLUSION: The methanolic rhizome extract of S. wallisii demonstrated the highest of pathogenic bacterial growth inhibition. This is the first report about the antibacterial activity of S. wallisii extracts that will add new information in natural drug discovery and development in industrial pharmacology.