Bioremediation by earthworms on soil microbial diversity and partial nitrification processes in oxytetracycline-contaminated soil.

A large proportion (60-90%) of ingested tetracyclines are released to slurry, soils, surface waters and ground water, which has raised extensive concerns and may pose a risk to the soil ecosystem. A 56-day experiment was conducted to study the bioremediation by earthworms on soil microbial diversity and partial nitrification processes in oxytetracycline (OTC)-contaminated soil. The results showed that high OTC concentration significantly decreased the activity of soil bacteria, ammonia-oxidizing bacteria (AOB) and archaea (AOA). Earthworms were found to accelerate the degradation efficiency and rate of OTC, and its main metabolites were 4-epi-oxytetracycline (EOTC) and 2-acetyl-2-decarboxamido-oxytetracycline (ADOTC). Earthworms had an important role in the bioremediation of soil microbial diversity by degrading OTC and its metabolite (EOTC), especially in the high OTC condition. Additionally, the results indicated that the effects of earthworms on the degradation of OTC could remediate the abundances of 16S rRNA and AOB amoA genes and the NO3- content in both low and high OTC-contaminated soils. The structural equation model suggested that earthworms could remediate the microbial diversity, the abundances of 16s rRNA and AOB amoA genes by accelerating the degradation of OTC, which contributed to the bioremediation by earthworms on soil microbial diversity and partial nitrification processes in oxytetracycline-contaminated soil.