[Dynamic changes and localization of plasma heparin cofactor II before and after delivery in hypertensive pregnancy].

OBJECTIVE

To explore the physiological significance of the anticoagulation effect of heparin cofactor II (HCII) in vivo and investigate the pathogenesis of the development of thrombosis in hypertensive pregnancy.

METHODS

1. Plasma level of HCII activity was measured by chromogenic assay in 18 healthy adults, 18 normal pregnant women and 29 hypertensive pregnant women before and after delivery. 2. Plasma level of HCII antigen was measured by Western blot in 29 hypertensive pregnant women before and after delivery. 3. The distribution of HCII in the placenta was determined by immunofluorescent method.

RESULTS

The plasma HCII activity and antigen content in hypertensive pregnancy decreased significantly before delivery. They correlated with the severity of hypertensive pregnancy, and restored to normal after delivery. No HCII was found in normal and pathological placenta.

CONCLUSION

1. Plasma HCII activity decreased during pregnancy in hypertensive pregnancy. 2. The placenta is not the target of HCII. 3. Dynamic determination of plasma HCII level can reflect the severity of hypertensive pregnancy and be used as one of prognostic indices.