[Role of glomerular mesangial cell in the pathogenetic progress of lupus nephritis fibrosis].

OBJECTIVE

To explore the roles of proliferative lesions of mesangial cell and reactive oxygen species (ROS) in the pathogenetic progress of lupus nephritis fibrosis.

METHODS

Control mice C57BL/6 and model mice NZBWF1 were selected. Kidney tissues were collected for hematoxylin and eosin (HE) and Masson staining at Week 8, 12 and 16. The proliferation of glomerular cells was assessed by BrdU incorporation light microscopy. And the expression of NOX4 protein was detected by Western blot.

RESULTS

HE and Masson staining showed that model mice began to develop interstitial fibrosis and worsened at Weeks 12 and 16. The numbers of mesangial cell increased at Week 8 and became prominent at Weeks 12 and 16. The expression of NOX4 protein increased with the progression of lupus nephritis fibrosis.

CONCLUSION

Glomerular mesangial cell and ROS play an role in the pathogenetic progress of lupus nephritis fibrosis.