Marine drugs · 2019
Enzyme-Aided Extraction of Fucoidan by AMG Augments the Functionality of EPCs through Regulation of the AKT/Rheb Signaling Pathway.
СтатьяНазвание и аннотация: Перевод в очереди
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Rethineswaran Vinoth Kumar, Kim Yeon-Ju, Jang Woong Bi, Ji Seung Taek, Kang Songhwa, Kim Da Yeon, Park Ji Hye, Van Le Thi Hong, Giang Ly Thanh Truong, Ha Jong Seong, Yun Jisoo, Lee Dong Hyung, Yu Sun-Nyoung, Park Sul-Gi, Ahn Soon-Cheol, Kwon Sang-Mo
Аннотация
The purpose of the present study is to improve the endothelial progenitor cells (EPC) activation, proliferation, and angiogenesis using enzyme-aided extraction of fucoidan by amyloglucosidase (EAEF-AMG). Enzyme-aided extraction of fucoidan by AMG (EAEF-AMG) significantly increased EPC proliferation by reducing the reactive oxygen species (ROS) and decreasing apoptosis. Notably, EAEF-AMG treated EPCs repressed the colocalization of TSC2/LAMP1 and promoted perinuclear localization of mTOR/LAMP1 and mTOR/Rheb. Moreover, EAEF-AMG enhanced EPC functionalities, including tube formation, cell migration, and wound healing via regulation of AKT/Rheb signaling. Our data provided cell priming protocols to enhance therapeutic applications of EPCs using bioactive compounds for the treatment of CVD.
Предметные рубрики MeSH
ApoptosisCell MovementCell ProliferationCells, CulturedEndothelial Progenitor CellsGlucan 1,4-alpha-GlucosidaseHumansLysosomal-Associated Membrane Protein 1Neovascularization, PhysiologicPolysaccharidesProto-Oncogene Proteins c-aktReactive Oxygen SpeciesSignal TransductionTOR Serine-Threonine KinasesTuberous Sclerosis Complex 2 ProteinWound Healing