Antiproliferative effects of propofol and lidocaine on the colon adenocarcinoma microenvironment.

PURPOSE: Certain anesthetic interventions may influence the postoperative outcome in surgical cancer patients. Our study investigated the antiproliferative effects of propofol and lidocaine in two colon cancer cells lines, fibroblasts and in co-cultures.

METHODS

The antiproliferative effects of concentrations of propofol and lidocaine were assessed in HCT-116 and RKO cell lines, in fibroblasts (CCD-18Co) and in co-culture system.

RESULTS

Both propofol (2-4 mcg/ml) and lidocaine (2-4 µM) inhibited significantly colon cancer cell proliferation (p<0.05). Caspase-8, heat-shock proteins (HSP-27 and HSP-60), insulin growth factor (IGF)-II, insulin growth factor binding proteins, p53 protein and survivin were significantly differentially expressed in malignant cells and in fibroblasts exposed to lidocaine.

CONCLUSION

Lidocaine and propofol selectively inhibited colon cancer cells proliferation. Antiproliferative effects were tumor-, dose- and time-dependent and may be at least partially explained by activation of apoptosis protein pathways. Further studies are necessary to confirm the clinical impact of our data.