International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2025
MicroRNA whole-blood profiling in hospitalized patients with candidemia identified miR-125a-5p and miR-99b-5p as potential biomarkers for Candida albicans bloodstream infection.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ragozzino Silvio, Salete-Granado Daniel, Corchete Luis-Antonio, Vaquero-Herrero María-Paz, Bernardo Edgar, Siller-Ruiz María, Sánchez-González Rebeca, Castaño-Romero Fabián, Pérez-Nieto María-Ángeles, García-Señán Alicia, Gutiérrez-Cerrajero Carlos, Carbonell Cristina, Torres Jorge-Luis, González-Sarmiento Rogelio, Sabio Guadalupe, García-García Inmaculada, Ternavasio-de la Vega Hugo-Guillermo, Rojas-Pirela Maura, Marcos Miguel
Аннотация
OBJECTIVES: Analyze miRNA and mRNA expression in patients with bloodstream infection (BSI) caused by Candida albicans (CA) and non-albicans Candida spp. (CNA).
PATIENTS/METHODS: We prospectively enrolled 20 adults with candidemia (10 CA and 10 CNA) and 22 hospitalized controls without sepsis but with comparable comorbidities. miRNA and mRNA expression were determined by next-generation sequencing (NGS), and differentially expressed miRNAs were validated by qPCR. Integrated miRNA-mRNA and KEGG/Reactome enrichment analyses were used to predict miRNA targets and identify perturbed pathways.
RESULTS: NGS detected seven dysregulated miRNAs in CA vs controls. qPCR confirmed marked overexpression of miR-125a-5p and miR-99b-5p in CA compared with CNA and controls. No miRNAs were differentially expressed between the CNA and control groups. RNA-Seq revealed that 22, 111, and 152 genes were differentially expressed in CA vs controls, CNA vs controls, and CA vs CNA, respectively. Enrichment analysis highlighted cell-cycle and DNA-replication programs in both species groups, while CA uniquely affected histidine/phenylalanine metabolism. Integrated mapping linked upregulated miR-125a-5p to repression of pro-apoptotic and immune-modulatory genes, whereas miR-99b-5p targeted cell-cycle checkpoint genes.
CONCLUSIONS: Overexpression of miR-125a-5p and miR-99b-5p in whole blood may discriminate C. albicans candidemia. These miRNAs are promising rapid biomarkers and targets for antifungal therapy.
PATIENTS/METHODS: We prospectively enrolled 20 adults with candidemia (10 CA and 10 CNA) and 22 hospitalized controls without sepsis but with comparable comorbidities. miRNA and mRNA expression were determined by next-generation sequencing (NGS), and differentially expressed miRNAs were validated by qPCR. Integrated miRNA-mRNA and KEGG/Reactome enrichment analyses were used to predict miRNA targets and identify perturbed pathways.
RESULTS: NGS detected seven dysregulated miRNAs in CA vs controls. qPCR confirmed marked overexpression of miR-125a-5p and miR-99b-5p in CA compared with CNA and controls. No miRNAs were differentially expressed between the CNA and control groups. RNA-Seq revealed that 22, 111, and 152 genes were differentially expressed in CA vs controls, CNA vs controls, and CA vs CNA, respectively. Enrichment analysis highlighted cell-cycle and DNA-replication programs in both species groups, while CA uniquely affected histidine/phenylalanine metabolism. Integrated mapping linked upregulated miR-125a-5p to repression of pro-apoptotic and immune-modulatory genes, whereas miR-99b-5p targeted cell-cycle checkpoint genes.
CONCLUSIONS: Overexpression of miR-125a-5p and miR-99b-5p in whole blood may discriminate C. albicans candidemia. These miRNAs are promising rapid biomarkers and targets for antifungal therapy.