Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2010
Immediate early inflammatory gene responses of human umbilical vein endothelial cells to hemorrhagic venom.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Albrecht Eric A, Dhanasekaran Saravana M, Tomlins Scott
Аннотация
OBJECTIVE AND DESIGN: This report describes a focused immediate early gene response by human umbilical vein endothelial cells (HUVEC) to Echis carinatus snake venom.
MATERIALS OR SUBJECTS: Primary cultures of HUVEC were used to assess acute inflammatory gene responses.
TREATMENTS: Crude E. carinatus venom (2.5 µg/ml) was used to stimulate HUVEC.
RESULTS: HUVEC stimulated for 3 h with E. carinatus venom showed a focused response to the venom, with significant increases in metallothionein (e.g., MT1H, MT2A, MT1X) and cytochrome P450 (e.g., CYP1A1, CYP1B1) gene expressions compared to non-stimulated controls. Several other genes involved in cell growth and matrix attachment were repressed [e.g., thrombospondin 1 (THBS1), connective tissue growth factor (CTGF)].
CONCLUSIONS: These data suggest that acute vascular injury induced by hemorrhagic snake venom initiates an anti-oxidant response primarily involving metallothioneins.
MATERIALS OR SUBJECTS: Primary cultures of HUVEC were used to assess acute inflammatory gene responses.
TREATMENTS: Crude E. carinatus venom (2.5 µg/ml) was used to stimulate HUVEC.
RESULTS: HUVEC stimulated for 3 h with E. carinatus venom showed a focused response to the venom, with significant increases in metallothionein (e.g., MT1H, MT2A, MT1X) and cytochrome P450 (e.g., CYP1A1, CYP1B1) gene expressions compared to non-stimulated controls. Several other genes involved in cell growth and matrix attachment were repressed [e.g., thrombospondin 1 (THBS1), connective tissue growth factor (CTGF)].
CONCLUSIONS: These data suggest that acute vascular injury induced by hemorrhagic snake venom initiates an anti-oxidant response primarily involving metallothioneins.