Biomedical chromatography : BMC · 2016
Quantification of furosemide in camel plasma by high resolution mass spectrometry, application on pharmacokinetics.
СтатьяВалидационное исследованиеНазвание и аннотация: Перевод в очереди
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Wasfi Ibrahim A, Wajid Sayed A, Agha B A, Kamel Asmaa M, Al Biriki Nasreen A, Al Neaimi Khaled M, Al Ali Waleed A
Аннотация
We developed and validated a high-resolution liquid chromatography mass spectrometry method for the quantification of furosemide in camel plasma which was used for a pharmacokinetic study in camels. Plasma samples were extracted by supported liquid extraction and furosemide and internal standard (furosemide-D5) were separated on a an Agilent Zorbax XDB C18 column (50 × 2.1 mm i.d., 3.5 μm). Data was acquired in full-scan mode over a mass range of 200-400 Da in negative electrospray mode at a resolution of 70,000. Linear calibration curves were obtained over the concentration ranges of 1.0-10,000 ng/mL. The validated method was then successfully applied in evaluating the pharmacokinetics and metabolites of furosemide in six camels (Camelus dromedarus) and we were able to advice on a withdrawal time of furosemide treatment before racing.