American journal of reproductive immunology (New York, N.Y. : 1989) · 2004
Subpopulations of mouse testicular macrophages and their immunoregulatory function.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Bryniarski Krzysztof, Szczepanik Marian, Maresz Katarzyna, Ptak Maria, Ptak Włodzimierz
Аннотация
PROBLEM: Testicular macrophages (TMf) participate together with Sertoli cells in formation of blood-testis barrier. The present experiments were aimed to test their immunoregulatory functions in vivo and in vitro.
METHOD OF STUDY: TMf were purified by glass adherence, rosetting with opsonized erythrocytes and fractionation on discontinuous Percoll gradient (over 95% purity). Their antigen-presenting capacity in humoral and cell-mediated responses was tested in vitro (Mishell-Dutton cultures, proliferation assay) and in vivo (induction of contact sensitivity reaction).
RESULTS: TMf represent a heterogeneous cell population. Heavier Percoll fractions produce little transforming growth factor (TGF)-beta and are efficient antigen-presenting cells in humoral and cell-mediated immune responses. Lighter fractions produce high amounts of TGF-beta and are rather tolerogenic than immunogenic. Their immunosuppressive activity can be prevented by treatment of TMf donors with cyclophosphamide or in vitro by anti-TGF-beta monoclonal antibody. In non-separated TMf population the immunosuppressive activity prevails.
CONCLUSIONS: Subpopulation of TMf able to trigger specific immune responses is present in the testis but remains under control of other TMf subpopulation which minimizes the risk of development of autoimmune reactions.
METHOD OF STUDY: TMf were purified by glass adherence, rosetting with opsonized erythrocytes and fractionation on discontinuous Percoll gradient (over 95% purity). Their antigen-presenting capacity in humoral and cell-mediated responses was tested in vitro (Mishell-Dutton cultures, proliferation assay) and in vivo (induction of contact sensitivity reaction).
RESULTS: TMf represent a heterogeneous cell population. Heavier Percoll fractions produce little transforming growth factor (TGF)-beta and are efficient antigen-presenting cells in humoral and cell-mediated immune responses. Lighter fractions produce high amounts of TGF-beta and are rather tolerogenic than immunogenic. Their immunosuppressive activity can be prevented by treatment of TMf donors with cyclophosphamide or in vitro by anti-TGF-beta monoclonal antibody. In non-separated TMf population the immunosuppressive activity prevails.
CONCLUSIONS: Subpopulation of TMf able to trigger specific immune responses is present in the testis but remains under control of other TMf subpopulation which minimizes the risk of development of autoimmune reactions.