The Analyst · 2014
Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Liu Yang, Zhu Fanjiao, Dan Wangxia, Fu Yu, Liu Shaoqin
Аннотация
A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode surface. The binding of tumor cells to EpCAM antibodies causes increase of the electron-transfer resistance. The electrochemical impedance of the prepared biosensors is linear with the logarithm of concentration of the liver cancer cell line (HepG2) within the concentration range of 10 to 10(5) cells per mL. The detection limit for HepG2 cells is 5 cells per mL. The proposed impedimetric sensing devices allow for sensitive and specific detection of cancer cells in whole-blood samples without any sample pretreatment steps.
Предметные рубрики MeSH
AntibodiesAntibodies, ImmobilizedAntigens, NeoplasmBiosensing TechniquesBlood Chemical AnalysisCell Adhesion MoleculesElectric ImpedanceElectrodesEpithelial Cell Adhesion MoleculeFluorescent DyesHeLa CellsHep G2 CellsHumansImmunomagnetic SeparationMicroscopy, ConfocalNanotubes, CarbonNeoplastic Cells, CirculatingSpectrophotometry, UltravioletSurface PropertiesTin Compounds