Nature communications · 2021
Genetic fate-mapping reveals surface accumulation but not deep organ invasion of pleural and peritoneal cavity macrophages following injury.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Jin Hengwei, Liu Kuo, Tang Juan, Huang Xiuzhen, Wang Haixiao, Zhang Qianyu, Zhu Huan, Li Yan, Pu Wenjuan, Zhao Huan, He Lingjuan, Li Yi, Zhang Shaohua, Zhang Zhenqian, Zhao Yufei, Qin Yanqing, Pflanz Stefan, Kasmi Karim E I, Zhang Weiyi, Liu Zhaoyuan, Ginhoux Florent, Ji Yong, He Ben, Wang Lixin, Zhou Bin
Аннотация
During injury, monocytes are recruited from the circulation to inflamed tissues and differentiate locally into mature macrophages, with prior reports showing that cavity macrophages of the peritoneum and pericardium invade deeply into the respective organs to promote repair. Here we report a dual recombinase-mediated genetic system designed to trace cavity macrophages in vivo by intersectional detection of two characteristic markers. Lineage tracing with this method shows accumulation of cavity macrophages during lung and liver injury on the surface of visceral organs without penetration into the parenchyma. Additional data suggest that these peritoneal or pleural cavity macrophages do not contribute to tissue repair and regeneration. Our in vivo genetic targeting approach thus provides a reliable method to identify and characterize cavity macrophages during their development and in tissue repair and regeneration, and distinguishes these cells from other lineages.