Plug-and-play coaxial nested-tube supported liquid membrane extraction for the determination of nonsteroidal anti-inflammatory drugs in human plasma.

A robust coaxial nested-tube supported liquid membrane extraction (CNT-SLME) method was developed for determining nonsteroidal anti-inflammatory drugs (NSAIDs) in human plasma. The device was adapted from commercial nucleic acid purification columns using industrial-grade polypropylene fabric as a stable, hydrophobic support for a dihexyl ether liquid membrane. This "plug-and-play" three-phase system (acidic donor/organic membrane/alkaline acceptor) facilitates efficient analyte enrichment and reduced matrix interference. The setup allows simultaneous processing of multiple samples using a standard multi-well shaker, overcoming the low-throughput bottlenecks of traditional liquid-phase microextraction. Under optimized conditions, the method yielded satisfactory recoveries (46.89-66.63%), negligible matrix effects (86.50-103.51%), excellent linearity (R 2 > 0.998), and detection limits of 3.2-55.3 ng mL-1. Validation in spiked plasma demonstrated high accuracy (recoveries 90.05-104.69%) and precision (RSDs ≤ 8.26%). Given its simplicity, cost-effectiveness, and batch-processing capability, CNT-SLME provides a practical platform for routine therapeutic drug monitoring and clinical pharmacokinetic studies.