Reagentless uric acid biosensor based on Ni microdiscs-loaded NiO thin film matrix.

The development of a noninvasive test for uric acid has been the holy grail of uric acid detection research over the last decade. In the present work, a novel matrix comprising of a NiO thin film (a biocompatible material) loaded with Ni microdiscs was prepared on an ITO-coated glass substrate (Ni/NiO/ITO) with the help of RF sputtering for the reagentless detection of uric acid. The bioelectrode was fabricated by immobilizing uricase using a physical adsorption technique on the surface of the Ni/NiO/ITO electrode. The prepared matrix was found to be efficient in sensing biological processes occurring on the surface of the bioelectrode (Ur/Ni/NiO/ITO) in the presence of the analyte (uric acid) to obtain an electronic output. The biosensor exhibits a high sensitivity (431.09 μA mM(-1)), low Km value (0.15 mM), high apparent enzyme activity (5.07 × 10(-2) units per cm(2)), high shelf life (20 weeks) and good selectivity for the detection of uric acid over a wide concentration range (0.05 mM to 1 mM) without any external mediator in the PBS buffer. The obtained results are encouraging for the realization of a reagentless uric acid biosensor with efficient sensing response characteristics.