Molecules (Basel, Switzerland) · 2019
A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO2 Derivatives and Its Applications in Bioimaging.
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Qin Yan-Hong, Jiang Xiao-Yi, Que Yuan-Fang, Gu Jing-Yi, Wu Tong, Aihemaiti Ayinazhaer, Shi Ke-Xin, Kang Wen-Yu, Hu Bi-Ying, Lan Jin-Shuai, Ding Yue, Zhang Tong
Аннотация
Based upon the intramolecular charge transfer (ICT) mechanism, a novel ratiometric fluorescent probe EB was developed to detect SO32-/HSO3-. The probe displayed both colorimetric and ratiometric responses toward SO32-/HSO3-. It displayed a quick response (within 60 s), good selectivity and high sensitivity (a detection limit of 28 nM) towards SO32-/HSO3-. The SO32-/HSO3- sensing mechanism was confirmed as the Michael addition reaction by ESI-MS. Moreover, the probe could be applied to measure the level of sulfite in real samples, like sugar and chrysanthemum, and it could also be used to detect SO32-/HSO3- in HepG2 cells through confocal fluorescence microscopy, which proved its practical application in clinical diagnosis.