Rapid communications in mass spectrometry : RCM · 2025
Blood Collection Tube has Minimal Interference on the Carbon and Nitrogen Isotope Composition of Plasma, Red Blood Cells, and Serum in Cow Blood.
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Jaramillo David M, Bauman Lisa M, Nova Carlos Henrique Paiva Camisa, Lima Lais, van Santen Edzard
Аннотация
RATIONALE: Stable isotopes can be useful for tracking diet composition and other physiological processes in ruminants. These techniques rely on the fact that tissues directly reflect the animal's diet. Blood is a dynamic tissue that can be separated into serum, plasma, and red blood cell (RBC) components. Numerous collection tubes for blood are commercially available. No studies have compared the isotopic composition from cattle blood as affected by collection tube. This study compared commercially available serum and plasma tubes and evaluated how they may affect δ13C, δ15N, and C and N concentrations from blood in lactating dairy cattle.
METHODS: For plasma and RBC, treatments were five collection tubes containing different anticoagulants: dipotassium ethylenediaminetetraacetic acid (K2EDTA), tripotassium EDTA (K3EDTA), NaF/K oxalate, Li heparin (LiHep), and Na heparin (NaHep). For serum analysis, the three collection tubes were a RedTop (contained no coagulating agent) and TigerTop and Red+CAT, which both contained clot activator and serum separator gel, each made by two different manufacturers. All samples were collected via tail venipuncture in cows. All samples were processed, freeze dried, and analyzed in triplicate for δ13C, δ15N, and C and N.
CONCLUSIONS: While mean differences exist on the δ13C and δ15N from plasma and red blood cells, differences were minimal. Clot activator gels in serum tubes did not affect the δ13C and δ15N composition. This study showed that, while isotopic values may differ slightly from different blood collection tubes, these differences were often small, which may have little impact on the interpretation of research results when serum or RBC are analyzed. The isotopic composition of C and N concentrations of serum was not affected by the three tubes used within this study. Researchers will have to understand these differences and decide whether or not these may impact the objectives and the results of a given study.
METHODS: For plasma and RBC, treatments were five collection tubes containing different anticoagulants: dipotassium ethylenediaminetetraacetic acid (K2EDTA), tripotassium EDTA (K3EDTA), NaF/K oxalate, Li heparin (LiHep), and Na heparin (NaHep). For serum analysis, the three collection tubes were a RedTop (contained no coagulating agent) and TigerTop and Red+CAT, which both contained clot activator and serum separator gel, each made by two different manufacturers. All samples were collected via tail venipuncture in cows. All samples were processed, freeze dried, and analyzed in triplicate for δ13C, δ15N, and C and N.
CONCLUSIONS: While mean differences exist on the δ13C and δ15N from plasma and red blood cells, differences were minimal. Clot activator gels in serum tubes did not affect the δ13C and δ15N composition. This study showed that, while isotopic values may differ slightly from different blood collection tubes, these differences were often small, which may have little impact on the interpretation of research results when serum or RBC are analyzed. The isotopic composition of C and N concentrations of serum was not affected by the three tubes used within this study. Researchers will have to understand these differences and decide whether or not these may impact the objectives and the results of a given study.