Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi · 2003
[Study on ribotyping of Lyme borreliosis spirochete in Guizhou province].
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Wang Ding-ming, Hao Qin, Cai Xing-he, Wan Kang-lin, Wang Zhao-xiao, Chen Jian
Аннотация
OBJECTIVE: To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy.
METHODS: Polymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing.
RESULTS: There were two genospecies in the strains: 20 strains belong to Borrelia valaisiana, 1 strain is Borelia sp.
CONCLUSION: Borrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B. valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.
METHODS: Polymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing.
RESULTS: There were two genospecies in the strains: 20 strains belong to Borrelia valaisiana, 1 strain is Borelia sp.
CONCLUSION: Borrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B. valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.