Ginekologia polska · 1998
[Alterations in the content of collagenous constituents of amniotic fluid in the course of EPH-gestosis].
Сравнительное исследованиеАннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Gogiel T, Bielecki D A, Bańkowski E
Аннотация
OBJECTIVES: EPH-gestosis may cause placental insufficiency and affect the metabolism of placenta and fetus. Various metabolites of amniotic fluid may reflect the fetal metabolism.
DESIGN: It was decided to compare the content and some biochemical features of hydroxyproline-containing collagenous constituents in the amniotic fluid derived from normal gestations and gestations affected by EPH-gestosis.
MATERIALS AND METHODS: Amniotic fluid was taken during labour (full-term gestations) by amniocentesis from 10 healthy women (control group) and 10 women with EPH-gestosis. UV-absorption spectra, protein and hydroxyproline were measured in non-dialyzed and dialyzed amniotic fluid. Hydroxyproline-containing collagenous constituents of amniotic fluid were chromatographed on Sephadex G-200.
RESULTS: Statistical differences in concentrations of total and non-dialyzable hydroxyproline between normal and gestotic amniotic fluid were not found. In contrast to that, gestotic amniotic fluid contains more dialyzable hydroxyproline (6.2 mg/ml) than normal fluid (4.5 mg/ml; p < 0.05). Molecular sieving on Sephadex G-200 shows that this difference is due to higher content of low molecular collagen degradation products.
CONCLUSION: Amniotic fluid, derived from pregnancies complicated by EPH-gestosis, contains higher amount of low molecular collagen degradation products than normal amniotic fluid. It is probably the result of more intensive degradation of fetal and placental collagen.
DESIGN: It was decided to compare the content and some biochemical features of hydroxyproline-containing collagenous constituents in the amniotic fluid derived from normal gestations and gestations affected by EPH-gestosis.
MATERIALS AND METHODS: Amniotic fluid was taken during labour (full-term gestations) by amniocentesis from 10 healthy women (control group) and 10 women with EPH-gestosis. UV-absorption spectra, protein and hydroxyproline were measured in non-dialyzed and dialyzed amniotic fluid. Hydroxyproline-containing collagenous constituents of amniotic fluid were chromatographed on Sephadex G-200.
RESULTS: Statistical differences in concentrations of total and non-dialyzable hydroxyproline between normal and gestotic amniotic fluid were not found. In contrast to that, gestotic amniotic fluid contains more dialyzable hydroxyproline (6.2 mg/ml) than normal fluid (4.5 mg/ml; p < 0.05). Molecular sieving on Sephadex G-200 shows that this difference is due to higher content of low molecular collagen degradation products.
CONCLUSION: Amniotic fluid, derived from pregnancies complicated by EPH-gestosis, contains higher amount of low molecular collagen degradation products than normal amniotic fluid. It is probably the result of more intensive degradation of fetal and placental collagen.