Pain reports · 2025
Cellular and molecular characterisation of the peripheral immune environment in migraine.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
O'Brien Jayden A, Heng Benjamin, Ahn Seong Beom, Staats-Pires Ananda, Wake Ashleigh, Wong Rebecca, Peek Aimie L, Meylakh Noemi, Ng Karl, Stark Richard, Macefield Vaughan G, McGuire Helen M, Henderson Luke A, Austin Paul J
Аннотация
INTRODUCTION: Peripheral immune dysfunction may be critically involved in the pathophysiology of migraine. Some evidence supports a role for peripheral T cells, monocytes, and humoral factors including kynurenine metabolites and cytokines, however a comprehensive picture has yet to emerge.
OBJECTIVE: This study sought to undertake a systematic assessment of the immune changes in episodic and chronic migraine across phases of the migraine cycle.
METHODS: Migraine patients in different phases of the migraine cycle with a confirmed diagnosis of episodic or chronic migraine and age- and sex-matched healthy controls were recruited. Peripheral blood was assessed for circulating immune cells, plasma proteins, and kynurenine pathway metabolites in a cross-sectional case-control design. Data were acquired using high-dimensional approaches including proteomics, single-cell mass cytometry, and imaging flow cytometry.
RESULTS: Plasma proteins related to increased cell-cell adhesion and altered enzymatic activity were increased in migraine. The migraine prodrome displayed a strong and distinct proinflammatory phenotype defined by increased platelet-neutrophil aggregation, quinolinic acid production, and matrix metalloproteinase-9 expression. Migraine patients in the attack phase instead expressed higher levels of cytokine receptors and phosphorylated transcription factors in Th17 cells, monocytes, natural killer cells, and B cells. T cells were shifted to a mobilised, recirculating phenotype across all migraine phases. Episodic and chronic migraine patients were only distinguished by subtle changes in T-cell phenotype.
CONCLUSION: Distinct proinflammatory peripheral signatures were detected between migraine phases, while few alterations distinguished episodic and chronic status. These data provide a resource that may aid in the identification of peripheral immune cells and mediators contributing to migraine attack onset.
OBJECTIVE: This study sought to undertake a systematic assessment of the immune changes in episodic and chronic migraine across phases of the migraine cycle.
METHODS: Migraine patients in different phases of the migraine cycle with a confirmed diagnosis of episodic or chronic migraine and age- and sex-matched healthy controls were recruited. Peripheral blood was assessed for circulating immune cells, plasma proteins, and kynurenine pathway metabolites in a cross-sectional case-control design. Data were acquired using high-dimensional approaches including proteomics, single-cell mass cytometry, and imaging flow cytometry.
RESULTS: Plasma proteins related to increased cell-cell adhesion and altered enzymatic activity were increased in migraine. The migraine prodrome displayed a strong and distinct proinflammatory phenotype defined by increased platelet-neutrophil aggregation, quinolinic acid production, and matrix metalloproteinase-9 expression. Migraine patients in the attack phase instead expressed higher levels of cytokine receptors and phosphorylated transcription factors in Th17 cells, monocytes, natural killer cells, and B cells. T cells were shifted to a mobilised, recirculating phenotype across all migraine phases. Episodic and chronic migraine patients were only distinguished by subtle changes in T-cell phenotype.
CONCLUSION: Distinct proinflammatory peripheral signatures were detected between migraine phases, while few alterations distinguished episodic and chronic status. These data provide a resource that may aid in the identification of peripheral immune cells and mediators contributing to migraine attack onset.