Journal of AOAC International · 2025
Determination of Ascorbyl Palmitate and Ascorbyl Stearate in Foods by LC-MS/MS.
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Choi Lai-Sheung, Chan Sau-Fong, Yeung Kin-Wai
Аннотация
BACKGROUND: Ascorbyl palmitate (AP) and ascorbyl stearate (AS), together called ascorbyl esters, are antioxidants that are used as food additives to prolong the shelf-life of foods by protecting against deterioration caused by oxidation.
OBJECTIVE: This article aimed to develop a simple solvent extraction and LC-MS/MS method for the determination of AP and AS in a broad range of food matrices.
METHODS: AP and AS in foods were extracted with methanol containing 0.5% (w/v) L-ascorbic acid and analyzed by liquid chromatography-tandem mass spectrometry in negative electrospray ionization and multiple reaction monitoring (MRM) mode.
RESULTS: The method was able to quantitatively detect AP and AS in different food matrices (canned drink, dessert, frozen fish, herbs, oils, infant formula/formulated products, etc.) with recoveries of AP and AS at 73-113% and 75-113%, respectively, and the corresponding relative standard deviations (RSDs) of 0.3-16.1% and 0.2-13.7%. The limits of quantitation were 0.23 mg/kg for AP and 0.14 mg/kg for AS. Method applicability was also demonstrated by the detection of AP in powdered infant formula purchased from local market.
CONCLUSIONS: A simple solvent extraction and LC-MS/MS method was developed, validated, and demonstrated for the quantitative determination of AP and AS in various foods.
HIGHLIGHTS: The validated method based on solvent extraction with LC-MS/MS detection offered a fast and simple method for the analysis of AP and AS in a wide range of foods.
OBJECTIVE: This article aimed to develop a simple solvent extraction and LC-MS/MS method for the determination of AP and AS in a broad range of food matrices.
METHODS: AP and AS in foods were extracted with methanol containing 0.5% (w/v) L-ascorbic acid and analyzed by liquid chromatography-tandem mass spectrometry in negative electrospray ionization and multiple reaction monitoring (MRM) mode.
RESULTS: The method was able to quantitatively detect AP and AS in different food matrices (canned drink, dessert, frozen fish, herbs, oils, infant formula/formulated products, etc.) with recoveries of AP and AS at 73-113% and 75-113%, respectively, and the corresponding relative standard deviations (RSDs) of 0.3-16.1% and 0.2-13.7%. The limits of quantitation were 0.23 mg/kg for AP and 0.14 mg/kg for AS. Method applicability was also demonstrated by the detection of AP in powdered infant formula purchased from local market.
CONCLUSIONS: A simple solvent extraction and LC-MS/MS method was developed, validated, and demonstrated for the quantitative determination of AP and AS in various foods.
HIGHLIGHTS: The validated method based on solvent extraction with LC-MS/MS detection offered a fast and simple method for the analysis of AP and AS in a wide range of foods.